Background: Diarrhoeal diseases remain one of the leading causes of childhood morbidity and mortality in most developing countries, with enteropathogenic Escherichia coli (EPEC). Typical and atypical EPEC strains differ in several characteristics. Typical EPEC, a leading cause of infantile diarrhea in developing countries, is rare in industrialized countries, where atypical EPEC seems to be a more important cause of diarrhea. Typical and atypical EPEC also differ in genetic characteristics, serotypes, and virulence properties. The aim of study was survey of prevalence of typical and atypical Enteropathogenic Escherichia coli obtained from children with diarrhea.Methods: In the present study, we collected 272 stool samples related to children with diarrhea that were referred to Division of Microbiology, School of Public Health, Tehran University of Medical Sciences. E.coli isolates, Enteropathogenic Escherichia coli isolates, were identified by using standard microbiological and biochemical tests. Subsequently, for identification of atypical and typical EPEC was survey of presence of eae and stx and bfp A genes by PCR method.Findings: Out of 272 samples, 156 isolates were identified as E.coli. 10 isolates identified as EPEC each of them was atypical.Conclusion: High prevalence EPEC in children is a threat to children in our country. The previous research has shown that atypical EPEC, a leading cause of infantile diarrhea, is more in industrialized countries but in present study, all EPEC were atypical.